Protein A Search Results


protein a g magnetic beads  (MedChemExpress)
99
MedChemExpress protein a g magnetic beads
Protein A G Magnetic Beads, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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steroidogenic factor 1 sf 1  (R&D Systems)
93
R&D Systems steroidogenic factor 1 sf 1
Steroidogenic Factor 1 Sf 1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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protein a sepharose beads  (Novus Biologicals)
90
Novus Biologicals protein a sepharose beads
A. and B. HeLa cells were transfected with PRDX2-V5 (A) or PRDX4-V5 (B) vector and WCL was incubated with purified GST or GST-HIF-1α fusion protein in the presence of <t>glutathione-Sepharose</t> beads, followed by immunoblot assays with anti-V5 antibody (upper panels) or Ponceau S staining (lower panels).
Protein A Sepharose Beads, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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rabbit anti hnf4α  (R&D Systems)
94
R&D Systems rabbit anti hnf4α
A. and B. HeLa cells were transfected with PRDX2-V5 (A) or PRDX4-V5 (B) vector and WCL was incubated with purified GST or GST-HIF-1α fusion protein in the presence of <t>glutathione-Sepharose</t> beads, followed by immunoblot assays with anti-V5 antibody (upper panels) or Ponceau S staining (lower panels).
Rabbit Anti Hnf4α, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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recombinant human surfactant protein d sp d  (R&D Systems)
92
R&D Systems recombinant human surfactant protein d sp d
A. and B. HeLa cells were transfected with PRDX2-V5 (A) or PRDX4-V5 (B) vector and WCL was incubated with purified GST or GST-HIF-1α fusion protein in the presence of <t>glutathione-Sepharose</t> beads, followed by immunoblot assays with anti-V5 antibody (upper panels) or Ponceau S staining (lower panels).
Recombinant Human Surfactant Protein D Sp D, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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control staphylococcal recombinant protein fnbpa476  (Biosynth Carbosynth)
86
Biosynth Carbosynth control staphylococcal recombinant protein fnbpa476
A. and B. HeLa cells were transfected with PRDX2-V5 (A) or PRDX4-V5 (B) vector and WCL was incubated with purified GST or GST-HIF-1α fusion protein in the presence of <t>glutathione-Sepharose</t> beads, followed by immunoblot assays with anti-V5 antibody (upper panels) or Ponceau S staining (lower panels).
Control Staphylococcal Recombinant Protein Fnbpa476, supplied by Biosynth Carbosynth, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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neuropilin 1  (Boster Bio)
90
Boster Bio neuropilin 1
A. and B. HeLa cells were transfected with PRDX2-V5 (A) or PRDX4-V5 (B) vector and WCL was incubated with purified GST or GST-HIF-1α fusion protein in the presence of <t>glutathione-Sepharose</t> beads, followed by immunoblot assays with anti-V5 antibody (upper panels) or Ponceau S staining (lower panels).
Neuropilin 1, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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protein a agarose beads  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc protein a agarose beads
A. and B. HeLa cells were transfected with PRDX2-V5 (A) or PRDX4-V5 (B) vector and WCL was incubated with purified GST or GST-HIF-1α fusion protein in the presence of <t>glutathione-Sepharose</t> beads, followed by immunoblot assays with anti-V5 antibody (upper panels) or Ponceau S staining (lower panels).
Protein A Agarose Beads, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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protein a magnetic beads  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc protein a magnetic beads
A. and B. HeLa cells were transfected with PRDX2-V5 (A) or PRDX4-V5 (B) vector and WCL was incubated with purified GST or GST-HIF-1α fusion protein in the presence of <t>glutathione-Sepharose</t> beads, followed by immunoblot assays with anti-V5 antibody (upper panels) or Ponceau S staining (lower panels).
Protein A Magnetic Beads, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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protein a dynabeads  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc protein a dynabeads
A. and B. HeLa cells were transfected with PRDX2-V5 (A) or PRDX4-V5 (B) vector and WCL was incubated with purified GST or GST-HIF-1α fusion protein in the presence of <t>glutathione-Sepharose</t> beads, followed by immunoblot assays with anti-V5 antibody (upper panels) or Ponceau S staining (lower panels).
Protein A Dynabeads, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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resource source identifier antibodies ha probe santa cruz biotechnology  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology resource source identifier antibodies ha probe santa cruz biotechnology
A. and B. HeLa cells were transfected with PRDX2-V5 (A) or PRDX4-V5 (B) vector and WCL was incubated with purified GST or GST-HIF-1α fusion protein in the presence of <t>glutathione-Sepharose</t> beads, followed by immunoblot assays with anti-V5 antibody (upper panels) or Ponceau S staining (lower panels).
Resource Source Identifier Antibodies Ha Probe Santa Cruz Biotechnology, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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protein a g agarose prebound  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology protein a g agarose prebound
A. and B. HeLa cells were transfected with PRDX2-V5 (A) or PRDX4-V5 (B) vector and WCL was incubated with purified GST or GST-HIF-1α fusion protein in the presence of <t>glutathione-Sepharose</t> beads, followed by immunoblot assays with anti-V5 antibody (upper panels) or Ponceau S staining (lower panels).
Protein A G Agarose Prebound, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


A. and B. HeLa cells were transfected with PRDX2-V5 (A) or PRDX4-V5 (B) vector and WCL was incubated with purified GST or GST-HIF-1α fusion protein in the presence of glutathione-Sepharose beads, followed by immunoblot assays with anti-V5 antibody (upper panels) or Ponceau S staining (lower panels).

Journal: Oncotarget

Article Title: PRDX2 and PRDX4 are negative regulators of hypoxia-inducible factors under conditions of prolonged hypoxia

doi: 10.18632/oncotarget.7142

Figure Lengend Snippet: A. and B. HeLa cells were transfected with PRDX2-V5 (A) or PRDX4-V5 (B) vector and WCL was incubated with purified GST or GST-HIF-1α fusion protein in the presence of glutathione-Sepharose beads, followed by immunoblot assays with anti-V5 antibody (upper panels) or Ponceau S staining (lower panels).

Article Snippet: Whole cell lysates were prepared in modified RIPA buffer [50 mM Tris-HCl (pH 7.5), 1 mM β-mercaptoethanol, 150 mM NaCl, 1% Igepal, and protease inhibitor cocktail] and incubated overnight with the following antibodies (catalog number and supplier): HIF-1α (sc-10790, Santa Cruz); V5 epitope (NB600-381, Novus Biologicals), HIF-2α (NB100-122, Novus Biologicals), or p300 (NB500-161, Novus Biologicals) in the presence of protein A Sepharose beads (NBP1-97240, Novus Biologicals).

Techniques: Transfection, Plasmid Preparation, Incubation, Purification, Western Blot, Staining